HISTOPATHOLOGICAL EFFECTS OF LOCAL AND SYSTEMIC OZONE TREATMENT IN EXPERIMENTAL TYMPANIC MEMBRANE PERFORATIONS

Methods: Traumatic perforation was created by using a 21 gauge syringe needle under the microscope and then animals were then divided into 4 equal groups. The first group was the control group and it healed spontaneously. 0.5 mL local ozone oil was dropped to external ear in second group; 1.0 mL (1 µg / mL) ozone gas was given by rectal way in third group; 0.5 mL local ozone oil and 1.0 mL (1 µg / mL) rectal ozone gas was given in fourth group for 10 days. On day 11th, the tympanic membranes of the rats were excised and examined for inflammation, fibroblast proliferation, vascular density, collagenization and epithelization.

Results: It was observed that fibroblast proliferation, collagenization and epithelization scores of local, local+rectal, rectal ozone treatment groups were more significant compared to the control group (p<0.05).

Conclusion: This study showed the contribitions of local and systemic ozone treatment to wound healing by increasing inflammation, fibroblast proliferation, collagenization, vascular density and epithelization in experimental tympanic membrane perforations of rats. Keywords : Trauma, tympanic membrane perforation,wounds, rats, ozone