THE EFFECT OF L-ORNITHINE ON THE INNER EAR OF RATS IN THE LIPOPOLYSACCHARIDE INDUCED ENDOTOXEMIA MODEL
1Department of ENT, University of Mersin, Mersin, Turkey2Department of Pathology, University of Mersin, Mersin, Turkey
3Department of Pharmacology, University of Mersin, Mersin, Turkey
4Department of Biochemistry, University of Mersin, Mersin, Turkey
5Department of Anesthesiology and Reanimation, University of Mersin, Mersin, Turkey Objectives; To investigate the relationship between ornithine treatment, p53 expression and the histopathologic differentials of rats inner ear in endotoxemia induced by lipopolysaccharide.
Material and methods; Twentyfour healthy rats were randomly divided into 4 groups. The first group, served as control, received intraperitoneal saline; the second group, intraperitoneal endotoxin; the third group, intraperitoneal ornithine and saline, and the fourth group received intraperitoneal ornithine ten minutes before endotoxin administration.
Results; Congestion and subepitelial edema were observed in stria vascularis, and leukocyte, plasma cell infiltration in the parts of membraneuos labyrinthitis of modiolar region in the cochlea of the second and the fourth group. Inflammation was not detected in the parts of vestibulocochlear and facial nerves in meatus acousticus internus. When we compared the plasma levels of nitrite/nitrate and MDA among groups, we found statistically significant differences: between groups 1 and 2, 1 and 3, 1 and 4, 2 and 3, 3 and 4. However, no significant difference was found in groups 2 and 4. Ornithine did not reduce the harmful effect of sepsis in rats. Immunohistochemically, p53 immunoreactive cells were not detected in any groups.
Conclusion; L-ornithine can not improve the manifestations of septic picture in the rat cochlea.
Keywords : Inner ear immunity, labyrinthitis, sepsis, endotoxin, hearing loss, endotoxemia