ANİ İŞİTME KAYBINDA NLR VE PLR PROGNOZU BELİRLEMEDE İŞE YARAR MI?

Ceyhun AKSAKAL ¹, MD;

¹Tokat Devlet Hastanesi, Kulak Burun Boğaz, Tokat, Turkey

Özet

Giriş: Bu çalışmanın amacı nötrofil/lenfosit oranı (NLR) ve platelet/lenfosit (PLR) oranının ani işitme kaybında prediktif bir marker olarak kullanılabilirliğini araştırmaktır.

Yöntem: Bu retrospektif, vaka kontrollü, klinik çalışmada kırk sekiz adet ani işitme kaybı tanısı konulmuş ve tedavisi yapılmış ve benzer tedavileri ani işitme kayıplı hasta ile, hastaneye rutin sağlık kontrolleri için başvurmuş sağlıklı 40 birey karşılaştırılmıştır. Aynı zamanda tedaviye cevap veren ve tedaviye cevap vermeyen ani işitme kayıplı hastalar karşılaştırılmış ve istatistiksel yöntemlerle değerlendirilmiştir. Hastaların tedaviden önce alınmış kan örneklerinden elde edilen sonuçlar ile sağlıklı bireylerin laboratuvar değerleri karşılaştırılmıştır. NLR, PLR, Beyaz küre miktarı ve alt grupları hasta grubu için ve kontrol grubu için ayrı ayrı hesaplanmıştır.

Bulgular: NLR ve PLR, ani işitme kayıplı hasta grubunda kontrol grubuna göre anlamlı derecede yüksek bulunmuştur (sırasıyla p<0,0001 ve p<0,01). Tedaviye cevap veren gruptaki NLR değerleri ile tedaviye cevap vermeyen grupta NLR değerleri istatistiksel olarak farklı değildir (p>0,05). Ancak PLR değerleri tedaviye cevap vermeyen grupta tedaviye cevap veren gruba göre önemli düzeyde daha yüksektir (p<0,05).

Sonuç: Ani işitme kaybında NLR ve PLR'nin yüksek bulunması hastalığın etiyolojisinde inflamatuar teoriyi desteklerken, NLR'nin bu hastalığın prognozunu göstermede etkisi yoktur. Buna karşın bu çalışmanın sonuçlarına göre PLR'nin zayıf prognostik değeri vardır.

Özet

Giriş

Idiopathic sudden sensorineural hearing loss (ISSNHL) is a sensorineural hearing loss that develops suddenly within three days and occurs in three consecutive frequencies in the audiogram. The annual incidence rate is 5-20/100,000. It is more common after 40 years of age [1]. In terms of etiology, retrocochlear pathologies are responsible in approximately 1% of the cases, while 15% are caused by Meniere's syndrome and autoimmune diseases. The etiology is unknown in 85% of the cases. The cases with unknown etiology are defined as idiopathic sudden sensorineural hearing loss. It is believed that this condition could be caused by the inflammatory process or vascular occlusion at the vascular level in cochlea [2]. The vascular feeding of the cochlea is provided by the labyrinthine artery. Higher incidence of the sudden hearing loss in patients with conditions such as hypertension and diabetes mellitus are associated with events such as vasospasm, thrombosis and hemorrhage at microvascular level, and this supports the vascular theory in sudden hearing loss. The role of viral agents in ISSNHL has also been discussed. Especially mumps virus, varicella zoster, Epstein Barr virus and cytomegalovirus are responsible for etiology [3,4]. However, studies show that sudden sensorineural hearing loss could also be linked with chronic inflammatory condition, one of the most important causes of microvascular ischemia [5,6].

White blood cells (WBC) and its subtypes have been described as markers of inflammatory events, especially in cardiovascular diseases and cerebrovascular events [7,8]. In recent years, the neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) have been used as predictors of many diseases as easy and cheap inflammatory response parameters [9]. NLR has been investigated as a marker for systemic inflammatory response, especially in cardiovascular diseases, atherosclerosis and cerebrovascular events [7,8]. It was found that PLR increase was only a poor marker in diseases such as atherosclerosis [10].

The aim of the present study was to investigate the usefulness of the NLR and PLR as predictive markers for sudden sensorineural hearing loss.

Giriş

Yöntem ve Gereçler

The study was approved by the local ethics committee and conducted in accordance with the ethical principles described by the Declaration of Helsinki.

This retrospective case-control study included patients who were treated for sudden sensorineural hearing loss in the period between April 2010 and April 2017 in Tokat State Hospital and healthy individuals who applied to our hospital for routine health checks (Control group). For the definition of sudden sensorineural hearing loss, the hearing loss which occurred in the last three days and hearing loss more than 30 dB in three consecutive frequencies in the audiogram were taken as criteria. Routine otologic examinations and hematological analyzes were performed during the patient admissions. In addition, the retrocochlear pathologies were evaluated with MRI.

Patients who were diagnosed with systemic diseases such as diabetes mellitus, hypertension, chronic kidney and liver disease, patients with arteriovenous thrombosis and patients younger than 18 years were excluded from the study. In addition, patients who had otosclerosis, Meniere's disease, retrocochlear pathology, active or chronic middle ear disease and internal or middle ear anomalies were excluded from the study. The control group consisted of healthy individuals who applied to the hospital for routine health checks, had no active or chronic disease, had no active or chronic internal or middle ear disease, and had normal hearing levels in pure tone audiometry (air-conduction hearing threshold 0-20 dB). Forty-eight patients and 40 healthy individuals who met the criteria were included in the study. Before treatment, the consent forms were taken from all patients. Blood samples were taken from all patients before the treatment. NLR was calculated by dividing the amount of neutrophils by the amount of lymphocytes, and PLR was calculated by dividing the amount of platelet by the amount of lymphocytes. The complete blood count was determined by a fully automatic blood cell counter (BC-6800, Mindray, Guangdong, China).

Before the treatment, pure tone audiometry test was performed on patients during their admission. The air and bone conduction levels in both ears were evaluated at 250 Hz, 500 Hz, 1 kHz, 2 kHz, 4 kHz and 8 kHz frequencies. After medical treatment, control audiometry was performed at the first month of admission. Siegel's criteria were used to evaluate the hearing levels of patients before and after the treatment (Table 1) [11]. According to this criteria, the patient group was divided into two subgroups as treatment responder (complete + partial + slight) and non-responder (no improvement). Based on the Siegel's criteria, 25 patients were concluded to be in the treatment responder group and 23 patients allocated to the treatment non-responder group.

Table 1: Siegel's criteria

All patients were treated with oral methylprednisolone at a dose of 1 mg/kg (Prednol L 16 mg tb® and 4 mg tb®, Mustafa Nevzat, Istanbul, Turkey). Steroid therapy was terminated in two weeks with gradual dose reduction.

Statistical Analyses
Mean, standard deviation, median, minimum and maximum values were given for the statistical definition of the groups. The Kolmogorov-Smirnov test was used to determine whether the groups had normal distribution. For comparison of the groups, independent t-test, which is a parametric test, was used for the variables with normal distribution. Mann-Whitney U test, which is a non-parametric counterpart of independent t-test, was used for variables with non-normal distribution. Significance of the difference between the groups was evaluated at p<0.05 level. All statistical analyses were performed using Medcalc software v.16.4.3 (Ostend, Belgium).

Yöntem ve Gereçler

Bulgular

The mean age of the patient and control groups were 49.7±14.4 and 47.8±12.5, respectively. Twenty-eight of the patients in the patient group were female and 20 were male. Twenty-three of the individuals in the control group were female while 17 were male. There was no significant difference between the two groups in terms of age and gender (Table 2).

Table 2: The Demographic and Clinical Characteristics of the Patients and Controls

The laboratory results of the patient and control groups were shown in Table 2. The mean NLR value was 4.22±2.52 in ISSNHL patients and 1.86±0.69 in control group. It was found that two groups were not significantly different for NLR (p< 0.0001) (Figure 1, Table-2). Neutrophil values were 6.88±2.68 in the patient group and 4.59±1.64 in the control group. A significant difference was found between the two groups for neutrophil values (p< 0.0001) (Table 2). Lymphocyte levels were significantly higher in control group (p< 0.0001) (Table 2). The mean PLR value was 151±87.2 in patient group and 106±41.0 in control group. PLR was significantly higher in patient group (p< 0.05) (Figure 2).

Büyütmek İçin Tıklayın Fig 1: Mean NLR values of the patient and control groups.

Büyütmek İçin Tıklayın Fig 2: Mean PLR values of the patient and control groups.

Mean NLR level was 3.76±2 in the treatment responder group and 4.74±2.98 in the treatment non-responder group (p> 0.05) (Table 3). Mean PLR level was 134±77 in treatment responder group and 171±94 in treatment non-responder group. Mean PLR was not different between treatment non-responder and treatment responder groups (p> 0.05) (Table 3). Although the neutrophil and platelet values were higher in treatment non-responder group compared to treatment responder group, difference was not significant (p> 0.05 and p> 0.05, respectively) (Table 3).

Table 3: Statistical data between the recovered and unrecovered groups.

Bulgular

Tartışma

The present study aimed to investigate a possible role of inflammation in ISSNHL using inflammation markers such as NLR and PLR and to determine the usefulness of NLR and PLT as markers to evaluate the prognosis of ISSNHL. Forty eight patients with unilateral ISSNHL treated with standard treatment modalities were evaluated. NLR was significantly higher in ISSNHL groups compared to healthy control. On the other hand, the group of patients who responded to the treatment was not significantly different from the group of patients who did not for inflammation markers such as NLR and PLR.

Although the acute or chronic inflammation, viral infections and vascular events are considered in the etiology of sudden sensorineural hearing loss, the precise causes have not been fully identified yet. Atherosclerotic microvascular circulatory disorders, inflammatory and infectious causes are the most frequently blamed factors for ISSNHL [12]. There is also viral theory about the possible causes of ISSNHL. According to this theory, the herpes simplex virus is involved in the etiology of ISSNHL. However, the inability to obtain adequate responses to antiviral treatment weakens this theory [13]. Because of the higher incidence of ISSNHL in diseases with impaired microcirculation such as hypertension and diabetes, the microvascular dysfunction has been brought to the foreground in the etiology [14]. Ciccone et al. found that the ISSNHL was more common in patients with increased carotid intima-media thickness [15]. However, in some other studies new markers linking chronic inflammation with cochlear injury were investigated [16]. The increased amount of neutrophils increases the production of cytokines such as IL-6, IL-8, and tumor necrosis factor-?, thereby playing a role in inflammation. Increased platelet numbers were also found to be associated with microvascular occlusion and perfusion defects [17,18]. In recent years, PLR and NLR have been used as a new thrombo-inflammatory markers in the evaluation of the prognosis and mortality of different cerebrovascular and cardiovascular diseases [19,20]. In some studies, use of NLR and PLR increases as markers for systemic inflammatory response in autoimmune diseases, acute pancreatitis and malignancies has been investigated [21-23].

Platelets are an important factor in the provision of thrombosis, coagulation, development and progression of atherosclerosis. Elevated PLR values accompanied with higher platelet count are thought to be secondary to vascular ischemia. Increased PLR values in extremity ischemia support this hypothesis [24]. A number of prognostic indicators have been investigated for ISSNHL for a long time. These include tinnitus, vertigo, grade of hearing loss, presence of diabetes and audiogram shape [25,26]. Recently, some studies have proposed certain blood test parameters of complete blood count test (CBC) such as NLR and PLR as markers for the prognosis of ISSNHL. These studies reported that low levels of NLR and PLR were associated with good prognosis of ISSNHL [16,17,27]. In line with the previous studies, NLR and PLR in ISSNHL group were higher compared to healthy control group in the present study. Interestingly, although NLR was higher in treatment non-responder group compared to the treatment responder group, the difference was not significant. Likewise, PLR values in the treatment responder and non-responder groups were not significantly different.

Recently, ISSNHL has been reported to be associated with chronic inflammation [28]. In a study conducted by Masuda et al., associations were reported between prognosis in the ISSNHL patients and high-sensitivity C-reactive protein (hsCRP) and tumor necrosis factor (TNF) [29]. Neutrophil and lymphocyte counts, NLR and PLR are parameters related to inflammation [30]. Seo et al. [17] suggested that ISSNHL induces systemic stress and that the resulting endothelial damage and inflammation increase NLR and PLR. Elevated NLR and PLR values of ISSNHL patients in the present study support a possible association between SSNHL and inflammation. On the other hand, although NLR value of the ISSNHL patient group who did not respond to the treatment was higher than that of ISSNHL patient group who responded to treatment, the difference was not significant. This finding shows that NLR is not an effective marker for determining the prognosis of ISSNHL.

Oral methylprednisolone was administered for the treatment of all patients. On the other hand, only systemic steroid was used in some studies dealing with the use of NLR and PLR to evaluate the prognosis of ISSNHL [16,31]. Some other studies used intratympanic dexamethasone in addition to systemic steroid for the treatment of patients with SSNHL [17,32]. Twenty-five of the 48 patients (52%) with SSNHL in the present study responded to treatment. In their study about the prognostic value of NLR and PLR in patients with SSNHL, Ulu et al. [16] found that 21 of the 47 patients (44.6%) had response to treatment. On the other hand, Seo et al. [17] used intratympanic dexamethasone in addition to systemic steroid for the treatment of 348 patients with SSNHL and achieved response in 78.6% of the patients. In another study, pentoxifylline and oral vitamin B1 (250 mg thiamine hydrochloride) and B6 (250 mg pyridoxine hydrochloride) were used along with systemic steroid for the treatment of patients with SSNHL, and a response rate of 59.2% was observed [33]. These findings showed that different treatment modalities could affect the success rates in recovery of patients with SSNHL.

Main limitations of the present study were its retrospective nature and relatively limited number of patients included. There were only 48 patients meeting the study criteria in the eight-year period covered by the study based on medical records. Prospective studies with larger cohorts could be more useful for evaluating the prognostic value of NLR and PLR in SSNHL.

Tartışma

Sonuç

While the NLR and PLR increases in ISSNHL support the inflammatory theory in ISSNHL, NLR is not an effective marker for prognosis. However, PLR can be used as a marker, albeit a weak one, to determine prognosis.

Financial Disclosure: The authors declare that the present study received no financial support. Conflict of Interest: The authors have no conflicts of interest to declare.

Sonuç