WHAT ARE THE MULTIFACTORIAL HISTOPATHOLOGIC FEATURES OF EUSTACHIAN TUBE DAMAGE CAUSED BY CONTENTS OF THE STOMACH?
1Afyon Sandıklı Devlet Hastanesi, KBB Kliniği, Afyon, Türkiye2Süleyman Demirel Üniversitesi Tıp Fakültesi, KBB BBC AD, Isparta, Türkiye
3Adnan Menderes Üniversitesi, Tıp Fakültesi, Histoloji AD, Aydın, Türkiye Objective: The purpose of this study is to evaluate the histopathological characteristics of Eustachian tube damage caused by experimentally induced reflux in rats.
Methods: Total of 19 albino rats were divided into two groups according to being exposed to HCl/Pepsin and salin. Nasopharyngeal tube inserted to all rats through left nasal cavity for administration of HCl/Pepsin and salin. Five parameters were compared among the groups (goblet cell density, submucosal inflammatory cell infiltration, hypertrophy and depredation level of the submucosal glands and fibrous tissue proliferation) according to side of ear and nasopharyngeal or tympanic localization of Eustachian tube. Mann-Whitney U and Wilcoxon tests were used to compare the parameters.
Results: There was no difference with respect to goblet cell density between the groups. The other parameters were higher in HCL-pepsin group than control group (submucosal inflammatory tissue density, submucosal gland damage, submucosal gland hypertrophy and fibrous tissue proliferation). In addition, several inflammatory parameters were significantly higher in the nasopharynx localization compared to the timpanum localization.
Conclusion: In this study, we have shown that experimental contents of stomach (HCL-pepsin) which reached to nasopharynx cause significant multifactorial histopathological changes and harmless effects in Eustachian tube of rats.
Keywords : Gastroesophageal Reflux, Eustachian Tube, Middle Ear